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KMID : 1134820150440071079
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 7 p.1079 ~ p.1083
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Immuno-Modulatory Activities of Polysaccharides Separated from Jubak in Macrophage Cells
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Park Yoo-Young
Sung Nak-Yun
Byun Eui-Hong
Oh Kwang-Hoon
Byun Myung-Woo
Yoo Yung-Choon
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Abstract
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Activating macrophage cells play an important role in the host immune defense system. In this paper, immuno-modulatory activities of polysaccharides separated from Jubak (JPS) in macrophage cells were investigated. Immuno-modulatory activities were estimated based on cell proliferation, nitric oxide (NO) and cytokine production, degree of mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB phosphorylation in RAW264.7 macrophage cells. JPS (62.5 to 250 μg/mL) did not induce a cytotoxic event. Additionally, NO and proinflammatory cytokines (tumor necrosis factor-α and interleukin-6) production significantly increased in a dose-dependent manner. Similarly, phosphorylation of MAPKs and NF-κB increased upon JPS treatment. Therefore, our results suggest that polysaccharides separated from Jubak can induce macrophage activation through MAPK and NF-κB signaling and induction of Th1 polarization.
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KEYWORD
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Jubak polysaccharide, immuno-modulatory activity, macrophage activation, nitric oxide production, cytokine production
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